期刊
BIORESOURCE TECHNOLOGY
卷 99, 期 13, 页码 5459-5465出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2007.11.009
关键词
fibrous membrane; electrospinning; enzyme immobilization; lipase; membrane bioreactor
A simple way of fabricating enzymatic membrane reactor with high enzyme loading and activity retention from the conjugation between nanofibrous membrane and lipase was devised. Poly(acrylonitrile-co-2-hydroxyethyl methacrylate) (PANCHEMA) was electrospun into fibrous membrane and used as support for enzyme immobilization. The hydroxyl groups on the fibrous membrane surface were activated with epichlorohydrin, cyanuric chloride or p-benzoquinone, respectively. Lipase from Candida rugosa was covalently immobilized on these fibrous membranes. The resulted bioactive fibrous membranes were examined in catalytic efficiency and activity for-hydrolysis. The observed enzyme loading on the fibrous membrane with fiber diameter of 80-150 mm was up to 1.6% (wt/wt), which was as thrice as that on the fibrous membrane with fiber diameter of 800-1000 nm. Activity retention for the immobilized lipase varied between 32.5% and 40.6% with the activation methods of hydroxyl groups. Stabilities of the immobilized lipase were obviously improved. In addition, continuous hydrolysis was carried out with an enzyme-immobilized fibrous membrane bioreactor and a steady hydrolysis conversion (3.6%) was obtained at a 0.23 mL/min flow rate under optimum condition. (C) 2007 Elsevier Ltd. All rights reserved.
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