期刊
BIOPROCESS AND BIOSYSTEMS ENGINEERING
卷 38, 期 2, 页码 399-405出版社
SPRINGER
DOI: 10.1007/s00449-014-1279-1
关键词
Polysaccharide; Ganoderma lucidum; Genetic engineering; alpha-Phosphoglucomutase (PGM); Biosynthetic genes
资金
- National Natural Science Foundation of China [31360495]
- Kunming University of Science and Technology [KKSY201226107]
- Department of Science and Technology of Yunnan Province [2012BA015]
- CNTC [110201201009 BR-03]
- Hongyun Honghe Tobacco (Group) Co. Ltd. [HYHH2012HX06]
This study aimed to improve the production of polysaccharide by engineering the biosynthetic pathway in Ganoderma lucidum through the overexpression of alpha-phosphoglucomutase (PGM) gene. PGM is responsible for the linkage between sugar catabolism and sugar anabolism. The effects of PGM gene overexpression on intracellular polysaccharide (IPS) content, extracellular polysaccharide (EPS) production and transcription levels of three genes encoding the enzymes involved in polysaccharide biosynthesis, including PGM, UDP-glucose pyrophosphorylase (UGP), and beta-1,3-glucan synthase (GLS), were investigated. The maximum IPS content and EPS production in G. lucidum overexpressing the PGM gene were 23.67 mg/100 mg dry weight and 1.76 g/L, respectively, which were higher by 40.5 and 44.3 % than those of the wild-type strain. The transcription levels of PGM, UGP and GLS were upregulated by 4.77-, 1.51- and 1.53-fold, respectively, in the engineered strain, suggesting that increased polysaccharide biosynthesis may result from a higher expression of those genes.
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