期刊
BIOPROCESS AND BIOSYSTEMS ENGINEERING
卷 35, 期 7, 页码 1081-1088出版社
SPRINGER
DOI: 10.1007/s00449-012-0691-7
关键词
2,3-Butanediol; K. pneumonia; K. oxytoca; Fermentation; Capsular polysaccharide
资金
- Industrial Strategic Technology Development Program [10035241]
- Ministry of Knowledge Economy (MKE, Korea)
Klebsiella is one of the genera that has shown unbeatable production performance of 2,3-butanediol (2,3-BD), when compared to other microorganisms. In this study, two Klebsiella strains, K. pneumoniae (DSM 2026) and K. oxytoca (ATCC 43863), were selected and evaluated for 2,3-BD production by batch and fed-batch fermentations using glucose as a carbon source. Those strains' morphologies, particularly their capsular structures, were analyzed by scanning electron microscopy (SEM). The maximum titers of 2,3-BD by K. pneumoniae and K. oxytoca during 10 h batch fermentation were 17.6 and 10.9 g L-1, respectively; in fed-batch cultivation, the strains showed the maximum titers of 50.9 and 34.1 g L-1, respectively. Although K. pneumoniae showed higher productivity, SEM showed that it secreted large amounts of capsular polysaccharide, increasing pathogenicity and hindering the separation of cells from the fermentation broth during downstream processing.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据