4.5 Article

Acetone-butanol-ethanol (ABE) fermentation using Clostridium acetobutylicum XY16 and in situ recovery by PDMS/ceramic composite membrane

期刊

BIOPROCESS AND BIOSYSTEMS ENGINEERING
卷 35, 期 7, 页码 1057-1065

出版社

SPRINGER
DOI: 10.1007/s00449-012-0721-5

关键词

Acetone butanol ethanol (ABE); Fermentation; Pervaporation; In situ recovery

资金

  1. 973 program of China [2011CB707405]
  2. National Natural Science Foundation of China [21106067]
  3. Qinglan Project of Jiangsu Province
  4. Commonweal research of CAF [201004001]
  5. The Six Talent Summit of Jiangsu Province [06-A-047]

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PDMS/ceramic composite membrane was directly integrated with acetone-butanol-ethanol (ABE) fermentation using Clostridium acetobutylicum XY16 at 37 A degrees C and in situ removing ABE from fermentation broth. The membrane was integrated with batch fermentation, and approximately 46 % solvent was extracted. The solvent in permeates was 118 g/L, and solvent productivity was 0.303 g/(L/h), which was approximately 33 % higher compared with the batch fermentation without in situ recovery. The fed-batch fermentation with in situ recovery by pervaporation continued for more than 200 h, 61 % solvent was extracted, and the solvent in penetration was 96.2 g/L. The total flux ranged from 0.338 to 0.847 kg/(m(2)/h) and the separation factor of butanol ranged from 5.1 to 27.1 in this process. The membrane was fouled by the active fermentation broth, nevertheless the separation performances were partially recovered by offline membrane cleaning, and the solvent productivity was increased to 0.252 g/(L/h), which was 19 % higher compared with that in situ recovery process without membrane cleaning.

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