期刊
BIOPROCESS AND BIOSYSTEMS ENGINEERING
卷 33, 期 9, 页码 1025-1031出版社
SPRINGER
DOI: 10.1007/s00449-010-0427-5
关键词
Guehomyces pullulans; beta-Galactosidase; Psychrotolerant yeast; Lactose hydrolysis; Antarctica
资金
- National Infrastructure of Natural Resources for Science 386 and Technology Program of China [2005DKA21209]
A psychrotolerant yeast Guehomyces pullulans 17-1 was isolated from sea sediment in Antarctica. It was found that it could yield both extracellular and cell-bound beta-galactosidase. After optimization of the medium and cultivation conditions, it was found that the yeast strain produced over 17.2 U mL(-1) of beta-galactosidase activity within 120 h at the flask level while the yeast strain produced over 25.3 U mL(-1) of beta-galactosidase activity within 144 h during the 2-L fermentation. This is the highest beta-galactosidase activity produced by the wild type yeast strains reported so far. However, the optimal pH and temperature for the crude beta-galactosidase were 4.0 and 50 A degrees C, respectively. Lactose could be converted into glucose and galactose and a large amount of reducing sugar could be released from milk under catalysis of the yeast culture.
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