期刊
BIOPROCESS AND BIOSYSTEMS ENGINEERING
卷 31, 期 3, 页码 183-191出版社
SPRINGER
DOI: 10.1007/s00449-008-0205-9
关键词
statins; HMG-CoA-reductase inhibitors; biocatalysis; asymmetric synthesis; alcohol dehydrogenase
The chemoenzymatic synthesis of the tert-butyl (S)-6-chloro-5-hydroxy-3-ketohexanoate is described. Our approach relies on a highly regio- and enantioselective reduction of a beta,delta-diketohexanoate ester catalysed by NADP(H)-dependent alcohol dehydrogenase of Lactobacillus brevis (LBADH). A detailed description of the scale-up of the enzymatic synthesis of the hydroxyketo ester is given which includes a scale-up of the substrate synthesis as well, i.e. the preparation of diketo ester on a 100 g scale. Furthermore, studies directed towards improving the co-catalyst [NADP(H)] consumption of the enzymatic key step by kinetic studies and application of a biphasic reaction medium were performed.
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