期刊
BIOPOLYMERS
卷 100, 期 4, 页码 402-407出版社
WILEY
DOI: 10.1002/bip.22246
关键词
AG73; Bubble liposome; gene delivery; syndecan; ultrasound imaging
资金
- Industrial Technology Research Grant Program from New Energy, Industrial Technology Development Organization (NEDO) of Japan [04A05010]
- Japan Society of the Promotion of Science of Japan [20300179]
- [18650146]
- Grants-in-Aid for Scientific Research [23500567, 20300179] Funding Source: KAKEN
Targeted gene delivery to neovascular vessels in tumors is considered a promising strategy for cancer therapy. We previously reported that Bubble liposomes (BLs), which are ultrasound (US) imaging gas-encapsulating liposomes, were suitable for US imaging and gene delivery. When BLs are exposed to US, the bubble is destroyed, creating a jet stream by cavitation, and resulting in the instantaneous ejection of extracellular plasmid DNA (pDNA) or other nucleic acids into the cytosol. We developed AG73 peptide-modified Bubble liposomes (AG73-BL) as a targeted US contrast agent, which was designed to attach to neovascular tumor vessels and to allow specific US detection of angiogenesis (Negishi et al., Biomaterials 2013, 34, 501-507). In this study, to evaluate the effectiveness of AG73-BL as a gene delivery tool for neovascular vessels, we examined the gene transfection efficiency of AG73-BLwith US exposure in primary human endothelial cells (HUVEC). The transfection efficiency was significantly enhanced if the AG73-BL attached to the HUVEC was exposed to US compared to the BL-modified with no peptide or scrambled peptide. In addition, the cell viability was greater than 80% after transfection with AG73-BL. These results suggested that after the destruction of the AG73-BLwith US exposure, a cavitation could be effectively induced by the US exposure against AG73-BL binding to the cell surface of the HUVEC, and the subsequent gene delivery into cells could be enhanced. Thus, AG73-BL may be useful for gene delivery as well as for US imaging of neovascular vessels. (C) 2013 Wiley Periodicals, Inc.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据