期刊
BIOPOLYMERS
卷 95, 期 8, 页码 550-558出版社
WILEY
DOI: 10.1002/bip.21628
关键词
small-angle X-ray scattering; protein folding; microsecond mixing
资金
- NIH [GM23303, GM54836]
- NSF [MCB0721312]
- U.S. Department of Energy, Basic Energy Sciences, Office of Science [DE-AC02-06CH11357]
- BioCAT (National Institutes of Health-supported Research Center) [RR-08630]
- U.S. DOE [DE-AC02-06CH11357]
Small-angle X-ray scattering (SAXS) is a powerful method for obtaining quantitative structural information on the size and shape of proteins, and it is increasingly used in kinetic studies of folding and association reactions. In this minireview, we discuss recent developments in using SAXS to obtain structural information on the unfolded ensemble and early folding intermediates of proteins using continuous-flow mixing devices. Interfacing of these micromachined devices to SAXS beamlines has allowed access to the microsecond time regime. The experimental constraints in implementation of turbulence and laminar flow-based mixers with SAXS detection and a comparison of the two approaches are presented. Current improvements and future prospects of microsecond time-resolved SAXS and the synergy with ab initio structure prediction and molecular dynamics simulations are discussed. (C) 2011 Wiley Periodicals, Inc. Biopolymers 95: 550-558, 2011.
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