期刊
ACS APPLIED MATERIALS & INTERFACES
卷 10, 期 9, 页码 7614-7625出版社
AMER CHEMICAL SOC
DOI: 10.1021/acsami.7b03328
关键词
electrospun silk fibroin scaffolds; hydroxyapatite particles; polydopamine; human adipose-derived mesenchymal stem cells; osteogenesis
资金
- Translational Research Center for Protein Function Control (TRCP) - Ministry of Science, ICT and Future Planning, Republic of Korea [2016R1A5A1004694]
- Yonsei University Future Leading Research Initiative [2016-22-0102]
- Institute for Basic Science [IBS-R026-D1]
The development of functional scaffolds with improved osteogenic potential is important for successful bone formation and mineralization in bone tissue engineering. In this study, we developed a functional electrospun silk fibroin (SF) nanofibrous scaffold functionalized with two-stage hydroxyapatite (HAp) particles, using mussel adhesive-inspired polydopamine (PDA) chemistry. HAp particles were first incorporated into SF scaffolds during the electrospinning process, and then immobilized onto the electrospun SF nanofibrous scaffolds containing HAp via PDA-mediated adhesive chemistry. We obtained two-stage HAp-functionalized SF nanofibrous scaffolds with improved mechanical properties and capable of providing a bone-specific physiological microenvironment. The developed scaffolds were tested for their ability to enhance the osteogenic differentiation of human adipose-derived mesenchymal stem cells (hADMSCs) in vitro and repair bone defect in vivo. To boost their ability for bone repair, we genetically modified hADMSCs with the transcriptional coactivator with PDZ-binding motif (TAZ) via polymer nanoparticle-mediated gene delivery. TAZ is a well-known transcriptional modulator that activates the osteogenic differentiation of mesenchymal stem cells (MSCs). Two-stage HAp-functionalized SF scaffolds significantly promoted the osteogenic differentiation of TAZ-transfected hADMSCs in vitro and enhanced mineralized bone formation in a critical-sized calvarial bone defect model. Our study shows the potential utility of SF scaffolds with nanofibrous structures and enriched inorganic components in bone tissue engineering.
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