期刊
BIOPOLYMERS
卷 94, 期 6, 页码 810-819出版社
WILEY
DOI: 10.1002/bip.21533
关键词
activity based profiling; substrate fingerprinting; phosphopeptide microarray; protein tyrosine phosphatases (PTPs); enzyme kinetics
资金
- Ministry of Education of the Singapore government [R 143 000 394 112]
Protein tyrosine phosphatases (PTPs) constitute a large family of enzymes that play key roles in Cell signaling Malfunctions of PTP activity have been linked to major human diseases including cancer One key aspect in PTP biology is the elucidation of roles of PTPs, as well as substrates they act on, in different cellular events Herein, a library of 144 putative peptide substrates against different PTPs was synthesized and immobilized onto a glass slide to generate the corresponding phosphopeptide microarray Subsequent screening of the microarray against various PTPs provided a distinctive and comparative substrate fingerprint against each PTP Several new substrates were identified, which might aid in the future design of potent and selective PTPs inhibitors The signal-decrease microarray assay used in our studies provided a facile and efficient way for high-throughput determination of kinetic constants for peptide/PTP interactions en masse Finally, our microarray results were independently verified by traditional microplate-based enzymatic assays (C) 2010 Wiley Periodicals, Inc Biopolymers (Pept Sci) 94 810 819, 2010
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