期刊
BIOPHYSICAL JOURNAL
卷 106, 期 4, 页码 875-882出版社
CELL PRESS
DOI: 10.1016/j.bpj.2014.01.005
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资金
- National Institutes of Health [GM082989]
- Career Award in the Biomedical Sciences from the Burroughs Wellcome Fund
- Rita Allen Foundation
- American Cancer Society
- EMBO
- ERC
- [SFB 902]
The centromeric histone H3 variant centromeric protein A (CENP-A), whose sequence is the least conserved among all histone variants, is responsible for specifying the location of the centromere. Here, we present a comprehensive study of CENP-A nucleosome arrays by cryo-electron tomography. We see that CENP-A arrays have different biophysical properties than canonical ones under low ionic conditions, as they are more condensed with a 20% smaller average nearest-neighbor distance and a 30% higher nucleosome density. We find that CENP-A nucleosomes have a predominantly crossed DNA entry/exit site that is narrowed on average by 8 degrees, and they have a propensity to stack face to face. We therefore propose that CENP-A induces geometric constraints at the nucleosome DNA entry/exit site to bring neighboring nucleosomes into close proximity. This specific property of CENP-A may be responsible for generating a fundamental process that contributes to increased chromatin fiber compaction that is propagated under physiological conditions to form centromeric chromatin.
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