期刊
BIOPHYSICAL JOURNAL
卷 107, 期 12, 页码 2941-2952出版社
CELL PRESS
DOI: 10.1016/j.bpj.2014.10.021
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资金
- Center for Theoretical Biological Physics [PHY-1427654]
- National Science Foundation [NSF-MCB-1214457]
- Welch Foundation [C-1792]
- Cancer Prevention Research Institute of Texas Scholars in Cancer Research - Cancer Prevention and Research Institute of Texas
- National Science Centre (Grant Sonata BIS)
- European Molecular Biology Organization [2757]
- Direct For Mathematical & Physical Scien
- Division Of Physics [1427654] Funding Source: National Science Foundation
- Division Of Physics
- Direct For Mathematical & Physical Scien [1308264] Funding Source: National Science Foundation
- Div Of Molecular and Cellular Bioscience
- Direct For Biological Sciences [1214457] Funding Source: National Science Foundation
Molecular dynamics simulations supplement single-molecule pulling experiments by providing the possibility of examining the full free energy landscape using many coordinates. Here, we use an all-atom structure-based model to study the force and temperature dependence of the unfolding of the protein filamin by applying force at both termini. The unfolding time-force relation tau(F) indicates that the force-induced unfolding behavior of filamin can be characterized into three regimes: barrier-limited low-and intermediate-force regimes, and a barrierless high-force regime. Slope changes of tau(F) separate the three regimes. We show that the behavior of tau(F) can be understood from a two-dimensional free energy landscape projected onto the extension X and the fraction of native contacts Q. In the low-force regime, the unfolding rate is roughly force-independent due to the small (even negative) separation in X between the native ensemble and transition state ensemble (TSE). In the intermediate-force regime, force sufficiently separates the TSE from the native ensemble such that tau(F) roughly follows an exponential relation. This regime is typically explored by pulling experiments. While X may fail to resolve the TSE due to overlap with the unfolded ensemble just below the folding temperature, the overlap is minimal at lower temperatures where experiments are likely to be conducted. The TSE becomes increasingly structured with force, whereas the average order of structural events during unfolding remains roughly unchanged. The high-force regime is characterized by barrierless unfolding, and the unfolding time approaches a limit of similar to 10 mu s for the highest forces we studied. Finally, a combination of X and Q is shown to be a good reaction coordinate for almost the entire force range.
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