期刊
SCIENCE
卷 361, 期 6409, 页码 1356-+出版社
AMER ASSOC ADVANCEMENT SCIENCE
DOI: 10.1126/science.aau0382
关键词
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资金
- National Science Foundation [NSF OCE-1313747, NSF-ANT-1043671, OCE 1538525, OCE 1638804]
- National Institute of Environmental Health Sciences [NIEHS P01-ES021921]
- U.S. Department of Energy Genomics Science program [DE-SC0008593, DE-SC0018344]
- Gordon and Betty Moore Foundation [GBMF3828, GBMF4960]
- Czech Science Foundation [18-13458S]
- National Institutes of Health (Chemical Biology Interfaces-University of California, San Diego) [5T32GM112584]
- Dickinson-McCrink Fellowship
- Natural Sciences and Engineering Research Council of Canada (NSERC-PDF)
- Simons Foundation Fellowship of the Life Sciences Research Foundation
- NATIONAL INSTITUTE OF ENVIRONMENTAL HEALTH SCIENCES [P01ES021921] Funding Source: NIH RePORTER
- NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES [T32GM112584] Funding Source: NIH RePORTER
- Division Of Ocean Sciences [1538525] Funding Source: National Science Foundation
Oceanic harmful algal blooms of Pseudo-nitzschia diatoms produce the potent mammalian neurotoxin domoic acid (DA). Despite decades of research, the molecular basis for its biosynthesis is not known. By using growth conditions known to induce DA production in Pseudo-nitzschia multiseries, we implemented transcriptome sequencing in order to identify DA biosynthesis genes that colocalize in a genomic four-gene cluster. We biochemically investigated the recombinant DA biosynthetic enzymes and linked their mechanisms to the construction of DA's diagnostic pyrrolidine skeleton, establishing a model for DA biosynthesis. Knowledge of the genetic basis for toxin production provides an orthogonal approach to bloom monitoring and enables study of environmental factors that drive oceanic DA production.
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