期刊
BIOPHYSICAL JOURNAL
卷 106, 期 8, 页码 1630-1637出版社
CELL PRESS
DOI: 10.1016/j.bpj.2013.11.4507
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资金
- Slovenian Research Agency
- Ad Futura, Slovene Human Resources Development and Scholarship Fund
- ERC
- BBSRC
- BBSRC [BB/E019668/1, BB/D010918/1] Funding Source: UKRI
- EPSRC [EP/H02994X/1] Funding Source: UKRI
- Biotechnology and Biological Sciences Research Council [BB/D010918/1, BB/E019668/1] Funding Source: researchfish
- Engineering and Physical Sciences Research Council [EP/D001846/1, EP/H02994X/1] Funding Source: researchfish
Using phase-separated droplet interface bilayers, we observe membrane binding and pore formation of a eukaryotic cytolysin,Equinatoxin II (EqtII). EqtII activity is known to depend on the presence of sphingomyelin in the target membrane and is enhanced by lipid phase separation. By imaging the ionic flux through individual pores in vitro, we observe that EqtII pores form predominantly within the liquid-disordered phase. We observe preferential binding of labeled EqtII at liquid-ordered/liquid-disordered domain boundaries before it accumulates in the liquid-disordered phase.
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