期刊
BIOPHYSICAL JOURNAL
卷 105, 期 1, 页码 L1-L3出版社
CELL PRESS
DOI: 10.1016/j.bpj.2013.05.029
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类别
资金
- Korber Foundation
We report on a fiber laser-based stimulated emission-depletion microscope providing down to similar to 20 nm resolution in raw data images as well as 15-19 nm diameter probing areas in fluorescence correlation spectroscopy. Stimulated emission depletion pulses of nanosecond duration and 775 nm wavelength are used to silence two fluorophores simultaneously, ensuring offset-free colocalization analysis. The versatility of this superresolution method is exemplified by revealing the octameric arrangement of Xenopus nuclear pore complexes and by quantifying the diffusion of labeled lipid molecules in artificial and living cell membranes.
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