期刊
CELLULAR PHYSIOLOGY AND BIOCHEMISTRY
卷 50, 期 5, 页码 1764-1778出版社
KARGER
DOI: 10.1159/000494794
关键词
Pig; LncRNAs; CircRNAs; IUGR; Metabolic syndrome
资金
- Sichuan Science & Tech Support Program (Pig Genetic Resources Exploitation and Utilization for the 13th Five-Year-Project) [16ZC2838]
- Chinese National Science & Tech Support Program [2015BAD03B01]
- earmarked fund for China Agriculture Research System [CARS-36-05B]
- China Scholarship Council (CSC)
Background/Aims: Intrauterine growth restriction (IUGR) is a risk factor for adult metabolic syndrome, but how this disease is regulated by lncRNAs and circRNAs remains elusive. Methods: Here, we employed adult IUGR and normal pigs as models to evaluate the expression of various global lncRNAs and circRNAs in pig livers using RNA-seq. Results: In total, we obtained 1,162 million raw reads of approximately 104.54 Gb high quality data. After a strict five-step filtering process, 3,368 lncRNAs were identified, including 300 differentially expressed lncRNAs (p < 0.05) in the IUGR group relative to the control group. The cis-regulatory analysis identified target genes that were enriched in specific GO terms and pathways (p < 0.05), including amino acid metabolism, oxidoreductase activity, PPAR signaling pathway, and insulin signaling pathway. These are closely related to the observed phenotypes of increased gluconeogenesis and impaired mitochondrial oxidative phosphorylation in adulthood of the IUGR group. Additionally, we also identified 403 circRNAs, of which 44 were differentially expressed (p < 0.05). Interestingly, our results identified ATF4-miR214-circRNA7964 and TCF7-miR22-3p-circRNA16347 as two competing endogenous networks, which were closely associated with the observed increase in hepatic gluconeogenesis in the IUGR group. Conclusion: Together, this study reveals a multitude of candidate lncRNAs and circRNAs involved in the development of IUGR pigs, which could facilitate further researches on the molecular mechanisms of metabolic syndrome.
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