期刊
BIOPHYSICAL JOURNAL
卷 102, 期 10, 页码 2353-2361出版社
CELL PRESS
DOI: 10.1016/j.bpj.2012.04.010
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资金
- National Science Foundation [MCB 0919974]
- Direct For Biological Sciences
- Div Of Molecular and Cellular Bioscience [0919974] Funding Source: National Science Foundation
We have investigated protein stability in an environment of Escherichia coli cytoplasm using coarse-grained computer simulations. To coarse-grain a small slide of E. coli's cytoplasm consisting of over 16 million atoms, we have developed a self-assembled clustering algorithm (CGCYTO). CGCYTO uses the shape parameter and asphericity as well as a parameter lambda (ranging from 0 to 1) that measures the covolume of a test protein and a macromolecule against the covolume of a test protein and a sphere of equal volume as that of a macromolecule for the criteria of coarse-graining a cytoplasmic model. A cutoff lambda(c) = 0.8 was chosen based on the size of a test protein and computational resources and it determined the resolution of a coarse-grained cytoplasm. We compared the results from a polydisperse cytoplasmic model (PD model) produced by CGCYTO with two other coarse-grained hard-sphere cytoplasmic models: 1), F70 model, macromolecules in the cytoplasm were modeled by homogeneous hard spheres with a radius of 55 angstrom, the size of Ficoll70 and 2), HS model, each macromolecule in the cytoplasm was modeled by a hard sphere of equal volume. It was found that the folding temperature T-f of a test protein (apoazurin) in a PD model is similar to 5 degrees greater than that in a F70 model. In addition, the deviation of T-f in a PD model is twice as much as that in a HS model when an apoazurin is randomly placed at different voids formed by particle fluctuations in PD models.
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