期刊
JOURNAL OF MATERIALS CHEMISTRY B
卷 6, 期 42, 页码 6767-6780出版社
ROYAL SOC CHEMISTRY
DOI: 10.1039/c8tb01373j
关键词
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资金
- Ministry of Human Resource Development (MHRD, Govt. of India)
- Department of Science & Technology (DST, Govt. of India) INSPIRE
- Department of Biotechnology (DBT, Govt. of India) [BT/PR7818/MED/32/279/2013]
Impaired wound healing is primarily associated with inadequate angiogenesis, repressed cell migration, deficient synthesis of extracellular matrix (ECM) component/growth factors, and altered inflammatory responses in the wound bed environment. Herein, we report a simple process for the fabrication of PCL nanofiber mats embedded with placental-derived bioactive molecules (PCL-sPEM) rich in growth factors for full-thickness cutaneous wound healing. The physicochemical attributes and biological composition of PCL-sPEM nanofiber mats delivered a nontoxic environment in vitro and significantly promoted the adhesion, infiltration, and proliferation of human fibroblasts/keratinocytes. Conditioned media extracted from PCL-sPEM nanofiber mats enhanced the migration potential of the cells (fibroblasts/keratinocytes) involved in wound healing due to the release of growth factors embedded in it. Further, PCL-sPEM nanofiber mats attracted, stimulated and supported vascularization as determined by the Chick Chorioallantoic Membrane (CAM) assay. Interestingly, critical skin wounds of rats treated with PCL-sPEM nanofiber mats facilitated improved wound closure with well-organized dermis and epidermis, which could be ascribed to prominent vascularization, augmented migration of human foreskin fibroblasts (HFFs) & human epidermal keratinocytes (HEKs), increased collagen synthesis and early re-epithelialization. Collectively, our results suggest that PCL-sPEM nanofiber mats embedded with growth factors could be a suitable matrix for treating critical full-thickness wounds.
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