期刊
BIOPHYSICAL JOURNAL
卷 101, 期 1, 页码 43-52出版社
CELL PRESS
DOI: 10.1016/j.bpj.2011.05.044
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资金
- Conselho Nacional de Desenvolvimento Cientifico e Tecnologico
- Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior
- Fundacao de Amparo a Pesquisa do Rio de Janeiro
We perform a detailed investigation of the force x deformation curve in tether extraction from 3T3 cells by optical tweezers. Contrary to conventional wisdom about tethers extracted from cells, we find that actin filaments are present within them, so that a revised theory of tether pulling from cells is called for. We also measure steady and maximum tether force values significantly higher than previously published ones for 3T3 cells. Possible explanations for these differences are investigated. Further experimental support of the theory of force barriers for membrane tube extension is obtained. The potential of studies on tether pulling force x deformation for retrieving information on membrane-cytoskeleton interaction is emphasized.
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