期刊
BIOPHYSICAL JOURNAL
卷 101, 期 6, 页码 1546-1554出版社
CELL PRESS
DOI: 10.1016/j.bpj.2011.08.013
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资金
- National Science Foundation [0959721]
- Damon Runyon Cancer Research Foundation [DRG 2040-10]
- Div Of Biological Infrastructure
- Direct For Biological Sciences [0959721] Funding Source: National Science Foundation
Spatial gradients in the behaviors of soluble proteins are thought to underlie many phenomena in cell and developmental biology, but the nature and even the existence of these gradients are often unclear because few techniques can adequately characterize them. Methods with sufficient temporal resolution to study the dynamics of diffusing molecules can only sample relatively small regions, whereas methods that are capable of imaging larger areas cannot probe fast timescales. To overcome these limitations, we developed and implemented time-integrated multipoint moment analysis (TIMMA), a form of fluorescence fluctuation spectroscopy that is capable of probing timescales down to 20 mu s at hundreds of different locations simultaneously in a sample. We show that TIMMA can be used to measure the diffusion of small-molecule dyes and fluorescent colloids, and that it can create spatial maps of the behavior of soluble fluorescent proteins throughout mammalian tissue culture cells. We also demonstrate that TIMMA can characterize internal gradients in the diffusion of freely moving proteins in single cells.
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