期刊
BIOPHYSICAL JOURNAL
卷 98, 期 4, 页码 534-542出版社
CELL PRESS
DOI: 10.1016/j.bpj.2009.10.044
关键词
-
类别
资金
- Agence Nationale de la Recherche
- Ligue Nationale contre le Cancer (Comite Ile-de-France)
- Association Francaise contre les Myopathies
- Comite National de la Recherche Scientifique
- program C'Nano (Region Ile-de-France)
- Association pour la Recherche sur le Cancer
Traction forces between adhesive cells play an important role in a number of collective cell processes Intercellular contacts, in particular cadherin-based intercellular junctions, are the major means of transmitting force within tissues. We investigated the effect of cellular tension on the formation of cadherin-cadherin contacts by spreading cells on substrates with tunable stiffness coated with N-cadherin homophilic ligands On the most rigid substrates, cells appear well-spread and present cadherin adhesions and cytoskeletal organization similar to those classically observed on cadherin-coated glass substrates However, when cells are cultured on softer substrates, a change in morphology is observed the cells are less spread, with a more disorganized actin network. A quantitative analysis of the cells adhering on the cadherin-coated surfaces shows that forces are correlated with the formation of cadherin adhesions. The stiffer the substrates, the larger are the average traction forces and the more developed are the cadherin adhesions. When cells are treated with blebbistatin to inhibit myosin II, the forces decrease and the cadherin adhesions disappear. Together, these findings are consistent with a mechanosensitive regulation of cadherin-mediated intercellular junctions through the cellular contractile machinery.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据