Significant Impact on Muscle Mechanics of Small Nonlinearities in Myofilament Elasticity

Important mechanisms in muscle contraction have recently been reevaluated based on analyses that rely on the assumption of linear myofilament elasticity. However, the present theoretical study shows that nonlinearity of this elasticity, even when so minor that it may be difficult to detect in experimental data, could have great impact on the interpretation of muscle mechanical experiments. This is illustrated by using simulated stiffness and strain-versus-force data for muscle fibers shortening at different constant velocities. There is substantial quantitative agreement, for this condition, between models with distributed myofilament compliance and models where the compliance of the myofilaments and the actomyosin cross-bridges are lumped together into two separate elastic elements acting in series. The data thus support the usefulness of the latter, simpler, type of model in the analysis. However, most importantly, the data emphasize the importance of caution before reevaluating fundamental mechanisms of muscle contraction based on analyses relying on the assumption of linear myofilament elasticity.