期刊
BIOPHYSICAL JOURNAL
卷 98, 期 4, 页码 543-551出版社
CELL PRESS
DOI: 10.1016/j.bpj.2009.11.001
关键词
-
类别
资金
- Landesstiftung Baden-Wurttemberg
- European Umon-STREP NanoCues
- Alfried-Krupp von Bohlen und Halbach-Stiftung
- Max-Planck-Society At Heidelberg
- Center for Modelling and Simulation in the Biosciences (BIOMS)
- Cluster of Excellence CellNetworks
Spatial patterning of biochemical cues on the micro- and nanometer scale controls numerous cellular processes such as spreading, adhesion, migration, and proliferation Using force microscopy we show that the lateral spacing of individual integrin receptor-ligand bonds determines the strength of cell adhesion For spacings >= 90 nm, focal contact formation was inhibited and the detachment forces as well as the stiffness of the cell body were significantly decreased compared to spacings <= 50 nm Analyzing cell detachment at the subcellular level revealed that rupture forces of focal contacts increase with loading rate as predicted by a theoretical model for adhesion clusters Furthermore, we show that the weak link between the intra- and extracellular space is at the intracellular side of a focal contact. Our results show that cells can amplify small differences in adhesive cues to large differences in cell adhesion strength
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据