期刊
EUROPEAN REVIEW FOR MEDICAL AND PHARMACOLOGICAL SCIENCES
卷 23, 期 17, 页码 7408-7418出版社
VERDUCI PUBLISHER
DOI: 10.26355/eurrev_201909_18849
关键词
Non-small cell lung cancer (NSCLC); LINC00342; MiR-203a-3p; Cell Growth; Metastasis
资金
- National Natural Science Foundation of China [81560318, 81860346]
- Natural Science Foundation of Guangxi Province [2017GXNSFA 98087]
- First Affiliated Hospital of Guangxi Medical University [2017031]
- Guangxi Medical And Health Suitable Technology Development Project [S2017037, 52017021, Z20180927, S201659]
OBJECTIVE: Non-small cell lung cancer, (NSCLC) is the main form of lung cancer, leading to major causes of cancer mortality. It is well known that IncRNAs may be involved in the pathogenesis of cancer, including NSCLC. The aim of this study was to provide a novel therapeutic target of LINC00342 for the therapy of NSCLC. PATIENTS AND METHODS: The expression of LINC00342 and miR-203a-3p was detected by quantitative Real Time-Polymerase Chain Reaction (qRT-PCR). Cell proliferation was measured using the MTT assay. Colony formation analysis was performed to count the number of colonies. Cell migration and invasion were measured by transwell. Online software DIANA tools were used to predict binding sites of LINC00342 and miR-203a-3p. Luciferase reporter assay was conducted to confirm the interaction between LINC00342 and miR-203a-3p. RESULTS: The expression of LINC00342 was increased in NSCLC tissues and cells compared with normal tissues and cells. Knockdown of LINC00342 suppressed cell proliferation, colony formation, migration, and invasion. LINC00342 regulated the expression of miR-203a-3p by targeting it directly. MiR-203a-3p was down-regulated in NSCLC tissues and cells compared with normal tissues and cells. Furthermore, LINC00342 promoted NSCLC cells proliferation, colony formation, migration, and invasion by depleting the expression of miR-203a-3p. CONCLUSIONS: This work implied that LINC00342 functions in NSCLC acting as an oncogene. Briefly. LINC00342 contributes to NSCLC cells growth and metastasis via targeting miR-203a-3p competitively.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据