期刊
FOOD & FUNCTION
卷 11, 期 3, 页码 2291-2298出版社
ROYAL SOC CHEMISTRY
DOI: 10.1039/c9fo02813g
关键词
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资金
- National Natural Science Foundation of China [81770837, 81601333, 81600687, 81701491, 81702831, 81600685]
- Jiangsu Provincial Medical Youth Talent [QNRC2016109]
- Jiangsu Provincial Key Research and Development Program [BE2018616]
- Six Talent Peaks Project in Jiangsu Province [YY-084]
- Nanjing Technological Development Program [201803013]
- Nanjing Medical Science and Technique Development Foundation [YKK17180, YKK16199]
- Science and Technology Development Foundation Item of Nanjing Medical University [2015NJMUZD060]
A large number of bioactive peptides derived from breast milk have been identified to be multifunctional having anti-inflammatory, immunoregulatory and antimicrobial activities. Here, we report that an endogenous peptide located at beta-casein 211-225 amino acid from human breast milk (hereafter called CAMP(211-225)) presents specific antimicrobial activity against pathogenic E. coli and Y. enterocolitica. CAMP(211-225) is a novel peptide that occurs at higher levels in preterm milk than in term milk. The minimal inhibitory concentrations (MIC) of CAMP(211-225) against E. coli and Y. enterocolitica are 3.125 mu g ml(-1) and 6.25 mu g ml(-1), respectively, and the antimicrobial activity of CAMP(211-225) was also confirmed by a disk diffusion assay. Further studies using fluorescence staining, scanning electron microscopy and a DNA-binding assay revealed that CAMP(211-225) kills bacteria through a membrane-disrupting mechanism, but not by binding to intracellular nucleic acids. Neonatal necrotizing enterocolitis (NEC) is a devastating gastrointestinal disease in neonatal intensive care units. In our study, CAMP(211-225) administration effectively reduced ileal mucosa damage in an experimental NEC mice model. These results suggest that the antimicrobial peptide CAMP(211-225) may have potential value in the prevention and treatment of neonatal infections.
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