期刊
BIOPHYSICAL JOURNAL
卷 97, 期 10, 页码 2854-2862出版社
CELL PRESS
DOI: 10.1016/j.bpj.2009.08.052
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资金
- French Agence Nationale de la Recherche [JC 2007, JC07-195504]
- European Commission [MRTN-CT-2006-035884]
- Korea Science and Engineering Foundation [ROA-2007-000-20027-0]
- Korea Ministry of Education, Science, and Technology
We present a polarimetric two-photon microscopy technique to quantitatively image the local static molecular orientational behavior in lipid and cell membranes. This approach, based on a tunable excitation polarization state complemented by a polarized readout, is easily implementable and does not require hypotheses on the molecular angular distribution such as its mean orientation, which is a main limitation in traditional fluorescence anisotropy measurements. The method is applied to the investigation of the molecular angular distribution in giant unilamellar vesicles formed by liquid-ordered and liquid-disordered micro-domains, and in COS-7 cell membranes. The highest order contrast between ordered and disordered domains is obtained for dyes locating within the membrane acyl chains.
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