期刊
BIOPHYSICAL JOURNAL
卷 96, 期 10, 页码 4132-4143出版社
CELL PRESS
DOI: 10.1016/j.bpj.2009.02.034
关键词
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类别
资金
- National Institutes of Health [R01 AR049425, R01 AR043396, R01 AR055611]
- U.S. Department of Energy
- Basic Energy Sciences
- Office of Science [W-31-109-ENG-38]
- NIH [RR-08630]
The subfragment 2/light meromyosin hinge region has been proposed to significantly contribute to muscle contraction force and/or speed. Transgenic replacement of the endogenous fast muscle isovariant hinge A (exon 15a) in Drosophila melanogaster indirect flight muscle with the slow muscle hinge B (exon 15b) allows examination of the structural and functional changes when only this region of the myosin molecule is different. Hinge B was previously shown to increase myosin rod length, increase A-band and sarcomere length, and decrease flight performance compared to hinge A. We applied additional measures to these transgenic lines to further evaluate the consequences of modifying this hinge region. Structurally, the longer A-band and sarcomere lengths found in the hinge B myofibrils appear to be due to the longitudinal addition of myosin heads. Functionally, hinge B, although a significant distance from the myosin catalytic domain, alters myosin kinetics in a manner consistent with this region increasing myosin rod length. These structural and functional changes combine to decrease whole fly wing-beat frequency and flight performance. Our results indicate that this hinge region plays an important role in determining myosin kinetics and in regulating thick and thin filament lengths as well as sarcomere length.
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