期刊
BIOPHYSICAL JOURNAL
卷 96, 期 12, 页码 5050-5059出版社
CELL PRESS
DOI: 10.1016/j.bpj.2009.03.023
关键词
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类别
资金
- Life Sciences Research Foundation
- Novartis (Basel, Switzerland)
In this work, we describe pin-hole array correlation imaging, a multipoint version of fluorescence correlation spectroscopy, based upon a stationary Nipkow disk and a high-speed electron multiplying charged coupled detector. We characterize the system and test its performance on a variety of samples, including 40 nm colloids, a fluorescent protein complex, a membrane dye, and a fluorescence fusion protein. Our results demonstrate that pin-hole array correlation imaging is capable of simultaneously performing tens or hundreds of fluorescence correlation spectroscopy-style measurements in cells, with sufficient sensitivity and temporal resolution to study the behaviors of membrane-bound and soluble molecules labeled with conventional chemical dyes or fluorescent proteins.
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