期刊
BIO-PROTOCOL
卷 10, 期 13, 页码 -出版社
BIO-PROTOCOL
DOI: 10.21769/BioProtoc.3665
关键词
Malaria; Rosettes; Wet mount; Microscope; Live intracellular parasite staining
类别
资金
- A*STAR [JCO-DP BMSI/15-800006-SIGN]
- Open Fund-Young Individual Research Grant by the National Medical Research Council, Ministry of Health, Singapore [OF-YIRG NMRC/OFYIRG/0070/2018]
- A*STAR
In malaria, rosetting phenomenon is a condition where a Plasmodium-infected erythrocyte stably adheres to at least an uninfected erythrocyte. This phenomenon that occurs in all species of human malaria parasite is likely to be an immune escape mechanism for the parasite. However, it has been associated with malaria pathogenesis, possibly by facilitating microvasculature occlusion along with direct endothelial cytoadherence by the infected erythrocytes. There are different microscopy-based techniques to visualize rosettes but neither of these techniques has yet to qualify as the official gold standard method. We have found that these techniques can be used interchangeably, provided that the conditions of the experiments are properly controlled. Here, we presented three methods as options for rosetting assay, i.e., the unstained wet mount technique, acridine orange based-fluorescence microscopy technique and Giemsa stained wet mount method, with preparation steps that enable consistent performance in rosetting experiments.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据