期刊
BIO-PROTOCOL
卷 10, 期 8, 页码 -出版社
BIO-PROTOCOL
DOI: 10.21769/BioProtoc.3584
关键词
Colonic epithelial cells; Fecal exosomes; Isolation; Ultracentrifugation; Characterization
类别
资金
- National Institute of Diabetes and Digestive and Kidney Diseases [RO1-DK-116306, RO1-DK-107739]
- Department of Veterans Affairs [BX002526, BX004476]
Exosomes secreted by colonic epithelial cells are present in feces and contain valuable epigenetic information, such as miRNAs, proteins, and metabolites. An in-depth study of this information is conducive to the diagnosis or treatment of relevant diseases. A crucial prerequisite of such a study is to establish an efficient isolation method, through which we can obtain a relatively more significant amount of exosomes from feces. This protocol is designed to effectively isolate a large number of exosomes from contaminants and other particles in feces by a combined method with fast filtration and sucrose density gradient ultracentrifugation. Exosomes generated by this method are suitable for further RNA, protein, and lipid analysis.
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