期刊
BIO-PROTOCOL
卷 10, 期 3, 页码 -出版社
BIO-PROTOCOL
DOI: 10.21769/BioProtoc.3508
关键词
Bacterial electrophysiology; Membrane potential; Bacterial detection; Anti-microbial resistance (AMR); Cell vitality assay; Cell biophysics
类别
资金
- Biotechnology and Biological Sciences Research Council (BBSRC)/Engineering and Physical Sciences Research Council (EPSRC) [BB/M017982/1]
Detecting live bacteria is an important task for antimicrobial susceptibility testing (AST) in the medical sector and for quality-monitoring in biological industries. Current methods for live-bacteria detection suffer limitations in speed or sensitivity. In a recent paper, we reported that electrical response dynamics in membrane potential enable single-cell rapid detection of live bacteria. The electrical response can be observed within a minute after electrical stimulation. Thus, it has potential in accelerating AST and the monitoring of biological samples. This method also enables experiments for biophysical and microbiological investigations into bacterial electrophysiology. With the hope that more researchers, scientists and engineers will use electrical stimulation for their assays, here we detail each step of the electrical stimulation experiment.
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