期刊
BIOPHYSICAL JOURNAL
卷 96, 期 1, 页码 L4-L6出版社
CELL PRESS
DOI: 10.1016/j.bpj.2008.09.031
关键词
-
类别
资金
- Japan Society for the Promotion of Science, Japan
- Ministry of Education, Culture, Sports, Science and Technology, Japan
- CREST Soft Nanomachine Project of Japan Science and Technology Agency, Japan
Adenosine triphosphate (ATP) turnover drives various processive molecular motors and adenosine diphosphate (ADP) release is a principal transition in this cycle. Biochemical and single molecule mechanical studies have led to a model in which a slow ADP release step contributes to the processivity of myosin-V. To test the relationship between force generation and ADP release, we utilized optical trapping nanometry and single molecule total internal reflection fluorescence imaging for simultaneous and direct observation of both processes in myosin-V. We found that ADP was released 69 +/- 5.3 ms after force generation and displacement of actin, providing direct evidence for slow ADP release. As proposed by several previous studies, this slow ADP release probably ensures processivity by prolonging the strong actomyosin state in the ATP turnover cycle.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据