期刊
BIOPHYSICAL JOURNAL
卷 95, 期 7, 页码 3400-3406出版社
BIOPHYSICAL SOC
DOI: 10.1529/biophysj.108.131482
关键词
-
类别
资金
- Engineering and Physical Sciences Research Council (EPSRC, UK)
- University of Nottingham's Interdisciplinary Doctoral Training Centre
Dynamic light scattering and Fourier transform infrared spectroscopy were used to study the formation of prefibrillar aggregates and fibrils of bovine pancreatic insulin at 60 degrees C and at pH 1. The kinetics of disintegration of the prefibrillar aggregates were also studied using these techniques after a quench to 25 degrees C. These experiments reveal that formation of prefibrillar aggregates is reversible under the solution conditions studied and show that it is possible to significantly reduce the nucleation (lag) times associated with the onset of fibril growth in bovine pancreatic insulin solutions by increasing the concentration of prefibrillar aggregates in solution. These results provide convincing evidence that less structured prefibrillar aggregates can act as. bril-forming intermediates.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据