期刊
DRUG DELIVERY
卷 27, 期 1, 页码 1165-1175出版社
TAYLOR & FRANCIS LTD
DOI: 10.1080/10717544.2020.1801891
关键词
Human amniotic extracellular matrix; biologic scaffolds; PLGA microspheres; endometrium regeneration
资金
- Medicine and Health Science and Technology Plan in Zhejiang Province [2018KY429]
- National Natural Science Foundation of China [81802587]
- Natural Science Foundation of Zhejiang Province [LYY18H310001]
The endometrial injury usually results in intrauterine adhesions (IUAs). However, there is no effective treatment to promote the regeneration of the endometrium currently. The decellularized amnion membrane (AM) is a promising material in human tissue repair and regeneration due to its biocompatibility, biodegradability, as well as the preservation of abundant bioactive components. Here, an innovative drug-delivering system based on human amniotic extracellular matrix (HAECM) scaffolds were developed to facilitate endometrium regeneration. The 17 beta-estradiol (E-2) loaded PLGA microspheres (E-2-MS) were well dispersed in the scaffolds without altering their high porosity. E(2)released from E-2-MS-HAECM scaffoldsin vitroshowed a decreased initial burst release followed with a sustained release for 21 days, which coincided with the female menstrual cycle. Results of cell proliferation suggested E-2-MS-HAECM scaffolds had good biocompatibility and provided more biologic guidance of endometrial cell proliferation except for mechanical supports. Additionally, the mRNA expression of growth factors in endometrial cells indicated that HAECM scaffolds could upregulate the expression of EGF and IGF-1 to achieve endometrium regeneration. Therefore, these advantages provide the drug-loaded bioactive scaffolds with new choices for the treatments of IUAs.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据