期刊
BIOPHYSICAL CHEMISTRY
卷 155, 期 1, 页码 29-35出版社
ELSEVIER
DOI: 10.1016/j.bpc.2011.02.004
关键词
DNA aptamer dimers; Guanine quadruplex; Thrombin; Circular dichroism; Differential scanning calorimetry; Temperature-gradient gel electrophoresis
资金
- Slovak Grant Agency [2/0038/09, 1/0153/09, 1/0794/10]
- Comenius University [UK/393/2009]
- SF EU [26220120021]
We have applied circular dichroism (CD), temperature-gradient gel electrophoresis (TGGE) and differential scanning calorimetry (DSC) to study the properties of novel bioengineered DNA aptamer dimers sensitive to fibrinogen (F) and heparin (H) binding sites of thrombin and compared them with canonical single stranded aptamer sensitive to fibrinogen binding site of thrombin (Fibri). The homodiiner (FF) and heterodimer (FH) aptamers were constructed based on hybridization of their supported parts. CD results showed that both FF and FH dimers form stable guanine quadruplexes in the presence of potassium ions like those in Fibri. The thermal stability of aptamer dimers was slightly lower compared to those of canonical aptamers, but sufficient for practical applications. Both FF and FH aptamer dimers exhibited a potassium-dependent inhibitory effect on thrombin-mediated fibrin gel formation, which was on average two-fold higher than those of canonical single stranded Fibri aptamers. (C) 2011 Elsevier B.V. All rights reserved.
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