Retinoic Acid-Loaded Poly(lactic-co-glycolic acid) Nanoparticle Formulation of ApoB-100-Derived Peptide 210 Attenuates Atherosclerosis

We developed a vaccine formulation containing ApoB derived P210 peptides as autoantigens, retinoic acid (RA) as IP: 11 93 14. 8 On Mo , 17 Aug 2020 0 :0 : 7 Copyright: Amer can Scientific Publishers an immune enhancer, both of which were delivered using PLGA nanoparticles. The formula was used to induce an Delivered by Ingenta immune response in 12-week-old male Apoe(-/-) mice with pre-existing atherosclerotic lesions. The nanotechnology plat-form PRINT (R) was used to fabricate PLGA nanoparticles that encapsulated RA inside and adsorbed the P210 onto the particle surface. In this study, we demonstrated that immunization of Apoe(-/)- mice with the formulation was able to considerably attenuate atherosclerotic lesions, accompanied by increased P210 specific IgM and another oxidized lipid derived autoantigen, M2AA, specific IgG autoantibodies, and decreased the inflammatory response, as compared to the P210 group with Freund's adjuvant. Our formulation represents an exciting technology to enhance the efficacy of the P210 vaccine.