Local intestinal immune response including NLRP3 inflammasome in broiler chicken infected withCampylobacter jejuniafter administration ofLactobacillus reuteriB1/1

The aim of this study was to evaluate the influence ofLactobacillus reuteriB1/1 on the local intestinal immune response in the cecum of chickens experimentally infected byC. jejuniCCM6189. Day-old chicken were divided into four groups: control (C),L. reuteriB1/1 (LR),C. jejuniCCM6189 (CJ), and combinedL. reuteriB1/1 + C. jejuniCCM6189 (LRCJ). Cecal samples were collected during necropsy 12, 24, 48 hours after infection byC. jejuni(relative expression of NLRP3, caspase-1, interleukins, TLR4) and 4 as well as 7 day post infection (relative percentage of CD4+, CD8+, IgM).L. reuteriB1/1 significantly stimulated gene expression of NLRP3 inflammasome and other selected molecules, thereby increasing the capacity of the immune system to react more effectively to the infection ofC. jejuniin chicken. In addition, an increase of IgM and the percentage of CD8+ influenced byL. reuteriwas observed.