期刊
BIOORGANIC & MEDICINAL CHEMISTRY LETTERS
卷 23, 期 6, 页码 1626-1630出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.bmcl.2013.01.087
关键词
Carbonic anhydrase; Beta-class enzyme; Anion; Enzyme inhibitor; Flaveria bidentis; Photosynthesis
资金
- FP7 EU project (Metoxia and Dynano)
- Accordo di Programma, CNR-MSE, PAR
Several beta-carbonic anhydrases (CAs, EC 4.2.1.1) are present in all land plants examined thus far. Here we report the first detailed biochemical characterization of one such isoform, FbiCA 1, from the C-4 plant Flaveria bidentis, which was cloned, purified and characterized as recombinant protein. FbiCA 1 has an interesting CO2 hydrase catalytic activity (k(cat) of 1.2 x 10(5) and k(cat)/K-m of 7.5 x 10(6) M-1 x s(-1)) and was moderately inhibited by most simple/complex inorganic anions. Potent FbiCA 1 inhibitors were also detected, such as trithiocarbonate, diethyldithiocarbamate, sulfamide, sulfamic acid, phenylboronic acid and phenylarsonic acid (K(I)s in the range of 4-60 mu M). Such inhibitors may be used as tools to better understand the role of various beta-CA isoforms in photosynthesis. (C) 2013 Elsevier Ltd. All rights reserved.
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