期刊
BIOORGANIC & MEDICINAL CHEMISTRY LETTERS
卷 23, 期 6, 页码 1732-1735出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.bmcl.2013.01.065
关键词
Aggregation; Amyloid; Circular dichroism; Fluorescent dye; Microwave synthesis
资金
- National Institute On Aging [R15AG038977]
Dye-binding assays that are used to evaluate anti-aggregation ability of small molecule inhibitors towards amyloids are known to be prone to false-positive effects due to spectral overlaps between the dye and the inhibitor. Aza-BODIPY dye, which has both excitation and emission maxima above 600 nm, exhibits a significant increase in its fluorescence intensity in the presence of soluble oligomers of A beta 1-42. These results indicate that aza-BODIPY could serve as a near-IR probe for detecting conformational changes of A beta 1-42 soluble oligomers in vitro, and it should eliminate false-positive effects that are associated with currently utilized thioflavin T-based dyes. In addition, a facile synthesis of aza-BODIPY has been developed, which might further expand the applications of this dye. (C) 2013 Elsevier Ltd. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据