期刊
BIOORGANIC & MEDICINAL CHEMISTRY LETTERS
卷 23, 期 11, 页码 3393-3395出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.bmcl.2013.03.064
关键词
Short chain dehydrogenase/reductase (SDR); Carbohydrates; Yarrowia lipolytica; Biooxidation; Bioreduction
资金
- Austrian BMWFJ
- BMVIT
- SFG
- Standortagentur Tirol
- ZIT through the Austrian FFG-COMET-Funding Program
Yarrowia lipolytica short chain dehydrogenase/reductase (YlSDR) was expressed in Escherichia coli, purified and characterized in vitro. The substrate scope for YlSDR mediated oxidation was investigated with alcohols and unprotected carbohydrates spectrophotometrically, revealing a preference for secondary compared to primary alcohols. In reduction direction, YlSDR was highly active on ribulose and fructose, suggesting that the enzyme is a mannitol-2-dehydrogenase. In order to explore substrate tolerance especially for space-demanding, lipophilic protecting groups, 5-O-trityl-D-ribitol and 5-O-trityl-alpha,beta-D-ribose were investigated as substrates: YlSDR oxidized 5-O-trityl-D-ribitol and 5-O-trityl-alpha,beta-D-ribose and reduced the latter at the expense of NADP(H). (C) 2013 Elsevier Ltd. All rights reserved.
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