期刊
BIOORGANIC & MEDICINAL CHEMISTRY LETTERS
卷 22, 期 10, 页码 3522-3526出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.bmcl.2012.03.073
关键词
2 '-Amino-LNA; Locked nucleic acid; Modified nucleosides; Primer extension; Triphosphate
资金
- Danish National Research Foundation
- Danish National Advanced Technology Foundation
- European Research Council under the European Union [268776]
- University of Queensland
- European Research Council (ERC) [268776] Funding Source: European Research Council (ERC)
The triphosphate of the thymine derivative of 2'-amino-LNA (2'-amino-LNA-TTP) was synthesised and found to be a good substrate for Phusion (R) HF DNA polymerase, allowing enzymatic synthesis of modified DNA encoded by an unmodified template. To complement this, 2'-amino-LNA-T phosphoramidites were incorporated into DNA oligonucleotides which were used as templates for enzymatic synthesis of unmodified DNA using either KOD, KOD XL or Phusion polymerases. 2'-Amino-LNA-T in the template and 2'-amino-LNA-TTP as a substrate both decreased reaction rate and yield compared to unmodified DNA, especially for sequences with multiple 2'-amino-LNA-T nucleotides. Crown Copyright (C) 2012 Published by Elsevier Ltd. All rights reserved.
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