Constituents of Dalbergia sissoo Roxb. leaves with osteogenic activity

One new isoflavone glucoside, caviunin 7-O-[beta-D-apiofuranosyl-(1 -> 6)-beta-D-glucopyranoside] (10) and a new itaconic derivative, (E)-4-methoxy-2-(3,4-dihydroxybenzylidene)-4-oxobutanoic acid (15) along with series of isoflavones and flavonols with their glucosides (1-9 and 11-14) and a lignan glucoside (16) were isolated from the ethanolic extract of Dalbergia sissoo leaves. The structures of these compounds were established on the basis of IR, UV, H-1 and C-13 NMR, DEPT, COSY, HSQC, HMBC and MS data. All compounds (1-16) were assessed for osteogenic activity in primary calvarial osteoblast cultures. Compounds 1-4 and 10 increased alkaline phosphatase activity and mineralization thus resulting in significant osteogenic activity. (C) 2011 Elsevier Ltd. All rights reserved.