期刊
BIOORGANIC & MEDICINAL CHEMISTRY LETTERS
卷 22, 期 23, 页码 7163-7168出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.bmcl.2012.09.067
关键词
C-terminal sequencing; ArgC digestion; Arg-selective scavenging; TMPP
资金
- Japan Society for the Promotion of Science (JSPS)
This Letter describes a method for enriching C-terminal peptide of protein for C-terminal sequence analysis. This method employs endopeptidase ArgC digestion and C-terminal peptide enrichment using m-aminophenylboronic acid-agarose as an arginine-capture material. The selectively recovered C-terminal peptide incorporates no artificial derivatization. Therefore, the widely used functional groups (e. g. alpha-NH2 and alpha-COOH) can be used for any necessary transformation. In this research, a TMPP mass tag was attached to the alpha-NH2 group to clarify the amino acid sequence of the C-terminal peptide. (C) 2012 Elsevier Ltd. All rights reserved.
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