期刊
MATERIALS TODAY BIO
卷 8, 期 -, 页码 -出版社
ELSEVIER
DOI: 10.1016/j.mtbio.2020.100078
关键词
3D printing; Tissue engineering; 3D printer; Additive manufacturing; Bombyx mori silk fiber
资金
- JSPS KAKENHI [18H01797]
- Adaptable and Seamless Technology Transfer Program Through Target-driven R&D (A-STEP) from the Japan Science and Technology Agency (JST)
- Business-Academia Collaborative Research Promotion Project from Kyotango City
Here, we investigated the usefulness of silk fibroin nanofibers obtained via mechanical grinding of degummed silkworm silk fibers as an additive in bioinks for extrusion three-dimensional (3D) bioprinting of cell-laden constructs. The nanofibers could be sterilized by autoclaving, and addition of the nanofibers improved the shear thinning of polymeric aqueous solutions, independent of electric charge and the content of cross-linkable moieties in the polymers. The addition of nanofibers to bioinks resulted in the fabrication of hydrogel constructs with higher fidelity to blueprints. Mammalian cells in the constructs showed >85% viability independent of the presence of nanofibers. The nanofibers did not affect the morphologies of enclosed cells. These results demonstrate the great potential of silk fibroin nanofibers obtained via mechanical grinding of degummed silkworm silk fibers as an additive in bioinks for extrusion 3D bioprinting.
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