期刊
BIOORGANIC & MEDICINAL CHEMISTRY LETTERS
卷 21, 期 1, 页码 303-306出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.bmcl.2010.11.013
关键词
F-19 magnetic resonance imaging; Paramagnetic relaxation enhancement; Gd-DOTA; Nucleic acids
资金
- Ministry of Education, Science, Sports and Culture of Japan
To image gene expression in vivo, we designed and synthesized a novel signal turn-on probe for F-19 nuclear magnetic resonance (MR) imaging based on paramagnetic relaxation enhancement. The stem-loop structured oligodeoxyribonucleotide (ODN) having a molecular beacon sequence for point mutated K-ras mRNA was doubly labeled with bis(trifluoromethyl)benzene moiety and Gd-1,4,7,10-tetraazacyclododecane-1,4,7,10-tetraacetic acid chelate moiety at the each termini of the ODN probe, respectively. We found that the F-19 MR signal of the bis(trifluoromethyl) benzene moiety tethered at the 5' termini of the probe turned on by the addition of complementary ODN. The probe has the potential to image gene expressions in vivo. (C) 2010 Elsevier Ltd. All rights reserved.
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