期刊
BIOORGANIC & MEDICINAL CHEMISTRY LETTERS
卷 19, 期 14, 页码 3779-3782出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.bmcl.2009.04.097
关键词
CSI microarray; FID; Small-molecule specificity; Label-free detection
资金
- NIH [GM069420, CA041986, CA078045]
- March of Dimes [FY07-511]
- USDA
- W.M. Keck Foundation
- Vilas Associate awards
- NSERC
Determining the sequence specifity of DNA binding molecules is a non-trivial task. Here we describe the development of a platform for assaying the sequence specificity of DNA ligands using label free detection on high density DNA microarrays. This is achieved by combining Cognate Site Identification (CSI) with Fluorescence Intercalation Displacement (FID) to create CSI-FID. We use the well-studied small molecule DNA ligand netropsin to develop this high throughput platform. Analysis of the DNA binding properties of protein- and small molecule-based libraries with CSI-FID will advance the development of genome-anchored molecules for therapeutic purposes. (C) 2009 Elsevier Ltd. All rights reserved.
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