4.5 Article

Selection of a DNA aptamer that binds 8-OHdG using GMP-agarose

期刊

BIOORGANIC & MEDICINAL CHEMISTRY LETTERS
卷 19, 期 13, 页码 3619-3622

出版社

PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.bmcl.2009.04.130

关键词

DNA aptamer; SELEX; 8-OHdG

资金

  1. Ministry of Education, Culture, Sports, Science and Technology, Japan [19021017]
  2. New Energy and Industrial Technology Development Organization (NEDO) of Japan [06B44019]
  3. Special Coordination Funds for Promoting Science and Technology
  4. Grants-in-Aid for Scientific Research [19021017] Funding Source: KAKEN

向作者/读者索取更多资源

DNA aptamers, which bind specific molecule, such as 8-OHdG, with high affinity were investigated using an in vitro selection strategy called systematic evolution of ligands by exponential enrichment (SELEX). However, 8-OHdG was difficult to immobilize on a carrier for SELEX. Therefore, a DNA aptamer binding to 8-OHdG was selected using GMP-agarose as an analogue from a library of about 4(60) random ssDNA sources. As a result, three aptamer candidates were selected. Among the selected DNA aptamers, the No. 22 DNA aptamer exhibited a high affinity for 8-OHdG. The dissociation constant, K-D, of No. 22 DNA aptamer was on the order of 0.1 mu mol/L. This result suggests that using an analogue will be a useful new SELEX method for obtaining various aptamers that are difficult to immobilize on a matrix. (C) 2009 Elsevier Ltd. All rights reserved.

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