期刊
BIOORGANIC & MEDICINAL CHEMISTRY LETTERS
卷 18, 期 23, 页码 6255-6258出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.bmcl.2008.09.093
关键词
RNA; Light-activated; Antisense; Oligonucleotide; Caged compound; Gene regulation; Gene expression; RNA bandage
资金
- NIH [GM083030, GM071339]
- Camille and Henry Dreyfus Foundation
RNA bandages' are composed of two 6-12-mer 2'-OMe RNA strands complementary to a mRNA target that are joined by a photocleavable linker. These tandem oligonucleotides typically exhibit much higher affinity for the mRNA than the individual strands. An RNA bandage with binding arms of different lengths and a 4-base gap blocked translation in vitro of GFP mRNA; subsequent near-UV irradiation restored translation. This provides a general method of photomodulating hybridization for a variety of oligonucleotide-based technologies. (C) 2008 Elsevier Ltd. All rights reserved.
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