Design powerful predictor for mRNA subcellular location prediction in Homo sapiens

Messenger RNAs (mRNAs) shoulder special responsibilities that transmit genetic code from DNA to discrete locations in the cytoplasm. The locating process of mRNA might provide spatial and temporal regulation of mRNA and protein functions. The situ hybridization and quantitative transcriptomics analysis could provide detail information about mRNA subcellular localization; however, they are time consuming and expensive. It is highly desired to develop computational tools for timely and effectively predicting mRNA subcellular location. In this work, by using binomial distribution and one-way analysis of variance, the optimal nonamer composition was obtained to represent mRNA sequences. Subsequently, a predictor based on support vector machine was developed to identify the mRNA subcellular localization. In 5-fold cross-validation, results showed that the accuracy is 90.12% for Homo sapiens (H. sapiens). The predictor may provide a reference for the study of mRNA localization mechanisms and mRNA translocation strategies.

Automated summary

The study obtained the optimal nonamer composition using binomial distribution and one-way analysis of variance, and developed a support vector machine predictor to identify mRNA subcellular localization with an accuracy of 90.12% for Homo sapiens. This predictor may be useful for studying mRNA localization mechanisms and translocation strategies.