期刊
THERANOSTICS
卷 11, 期 10, 页码 4992-5009出版社
IVYSPRING INT PUBL
DOI: 10.7150/thno.55006
关键词
DEHP; primordial follicle assembly; female germ cell; granulosa cell; single-cell transcriptome
资金
- National Nature Science Foundation of China [31970788]
- Taishan Scholar Construction Foundation of Shandong Province of China [ts20190946]
This study used single-cell RNA sequencing to reveal the gene expression dynamics of female germ and granulosa cells following DEHP exposure in mice. DEHP was found to impede follicle assembly progression in germ cells, inhibit proliferative activity in granulosa cells, disrupt transforming growth factor-beta signaling, and elevate DNA damage and apoptosis in both germ and somatic cells. These findings provide new insights into the reproductive toxicity of DEHP exposure and its impact on ovarian cell interactions.
Rationale: Accumulated evidence indicates that environmental plasticizers are a threat to human and animal fertility. Di (2-ethylhexyl) phthalate (DEHP), a plasticizer to which humans are exposed daily, can trigger reproductive toxicity by acting as an endocrine-disrupting chemical. In mammals, the female primordial follicle pool forms the lifetime available ovarian reserve, which does not undergo regeneration once it is established during the fetal and neonatal period. It is therefore critical to examine the toxicity of DEHP regarding the establishment of the ovarian reserve as it has not been well investigated. Methods: The ovarian cells of postnatal pups, following maternal DEHP exposure, were prepared for single cell-RNA sequencing, and the effects of DEHP on primordial follicle formation were revealed using gene differential expression analysis and single-cell developmental trajectory. In addition, further biochemical experiments, including immunohistochemical staining, apoptosis detection, and Western blotting, were performed to verify the dataset results. Results: Using single-cell RNA sequencing, we revealed the gene expression dynamics of female germ cells and granulosa cells following exposure to DEHP in mice. Regarding germ cells: DEHP impeded the progression of follicle assembly and interfered with their developmental status, while key genes such as Lhx8, Figla, and others, strongly evidenced the reduction. As for granulosa cells: DEHP likely inhibited their proliferative activity, and activated the regulation of cell death. Furthermore, the interaction between ovarian cells mediated by transforming growth factor-beta signaling, was disrupted by DEHP exposure, since the expression of GDF9, BMPR1A, and SMAD3 was affected. In addition, DNA damage and apoptosis were elevated in germ cells and/or somatic cells. Conclusion: These findings offer substantial novel insights into the reproductive toxicity of DEHP exposure during murine germ cell cyst breakdown and primordial follicle formation. These results may enhance the understanding of DEHP exposure on reproductive health.
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